| Kit Size | 100 assays (manual) / 400 assays (auto-analyser) |
|---|---|
| Shipping Temperature | Ambient |
| Storage Temperature | Short term stability: 2-8oC, |
| Stability | > 2 years under recommended storage conditions |
| Analyte | Phosphate |
| Assay Format | Spectrophotometer, Auto-analyser |
| Detection Method | Absorbance |
| Wavelength | 360 nm |
| Signal Response | Increase |
| Linear Range | 0.1 to 10 μg of phosphate per assay |
| Limit of Detection | 0.16 mg/L |
| Reaction Time | ~ 20 min |
| Application examples | Processed foods and drinks, bakery products, dairy products, waste water samples, cosmetics, pharmaceuticals and other materials (e.g. biological cultures, etc.) |
Megazyme Phosphate Assay Kit
The Phosphate Assay Kit is a rapid, simple, reliable and accurate method for the specific measurement and analysis of phosphate in foodstuffs, beverages and other materials. The phosphate detection reaction employs the defined chemical substrate 2-amino-6-mercapto-7-methylpurine ribonucleoside (MESG). In the presence of phosphate, the substrate is converted enzymatically by purine nucleoside phosphorylase (PNPase) to the products ribose 1-phosphate and 2-amino-6-mercapto-7-methylpurine. An application note describes the dephosphorylation reaction of phytic acid using phytase and alkaline phosphatase (sold separately), and subsequent phosphate detection using the reagents provided in K-PHOS.
Note for Content: The number of manual tests per kit can be doubled if all volumes are halved.
Advantages
- Chemically defined detection method
- All reagents stable for > 2 years after preparation
- Rapid reaction (~ 20 min)
- Mega-Calc™ software tool is available from our website for hassle-free raw data processing
- Standard included
- Suitable for manual and auto-analyser formats
