| Kit Size | 50 assays |
|---|---|
| Method | Spectrophotometric at 340nm |
| Reaction Time | ~ 30min |
| Detection Limit | 1-100% of sample weight |
| Application Examples | Flours, infant formula, animal feed, pet foods, plant materials (e.g. onion), food products and other materials |
| Method Recognition | This method is a modification of AOAC Method 999.03 and AACC Method 32-32.01 |
Megazyme Fructan HK Assay Kit, 50 assays
The Fructan HK test kit is suitable for the specific measurement and analysis of all fructo-oligosaccharides (reducing and non-reducing) and of fructan polysaccharides but is not suitable for the analysis of samples containing high levels of D-glucose, D-fructose, sucrose or maltose. Note that this is a modified version of AOAC method 999.03/AACC Method 32.32.01 (K-FRUC) in which the overall assay principle remains unchanged but detection is performed through the enzymatic hexokinase/glucose-6-phosphate dehydrogenase system as opposed to the reducing sugar PAHBAH determination. The K-FRUCHK procedure has not received official method status.
UV-method for the determination of Fructan in foodstuffs,
beverages and other materials
Principle:
(sucrase, pH 6.5, 40°C)
(1) Sucrose + H2O → D-glucose + D-fructose
(Maltase, pH 6.5, 40°C)
(2) maltose + maltotriose → D-glucose
(hexokinase + phosphoglucose isomerase)
(3) D-Glucose + D-fructose + ATP → G-6-P + ADP
(glucose-6-phosphate dehydrogenase)
(4) G-6-P + NADP+ → gluconate-6-phosphate + NADPH + H+
(exo-inulinase + endo-inulinase, pH 4.5, 40°C)
(5) Fructan + H2O → D-glucose + D-fructose
Advantages
- Very cost effective
- All reagents stable for > 12 months after preparation
- Fructan kits are available only from Megazyme
- Simple format
- Mega-Calc™ software tool is available from our website for hassle-free raw data processing
- Standard included
