| Kit Size | 100 assays |
|---|---|
| Method | Spectrophotometric at 510nm |
| Reaction Time | ~ 120min |
| Detection Limit | Amylose 5-95% of total starch content |
| Application Examples | Cereal starches, flours, pure starches and foods |
| Method Recognition | Novel method |
Megazyme Amylose/Amylopectin Assay Kit
The Amylose/Amylopectin test kit is suitable for the measurement and analysis of amylose/amylopectin ratio and content in cereal starches and flours. Based on a Con A precipitation procedure.
Colourimetric method for the determination of Amylose and
Amylopectin in cereals, food and feed
Principle:
(Con A)
(1) Soluble starch (amylose + amylopectin) →
amylose + amylopectin-Con A
(soluble) (precipitate)
(α-amylase + amyloglucosidase)
(2) Amylose (in solution) + H2O → D-glucose
(glucose oxidase)
(3) D-Glucose + H2O + O2 → D-gluconate + H2O2
(peroxidase)
(4) H2O2 + p-hydroxybenzoic acid + 4-aminoantipyrine →
quinoneimine + H2O
(α-amylase + amyloglucosidase)
(5) Total starch (in solution) + H2O → D-glucose
Advantages
- Very cost effective (cost per test)
- All reagents stable for > 12 months after preparation
- Only enzymatic kit available
- Accurate and reliable amylose / amylopectin ratio determination
- Simple format
- Standard included
