| Kit Size | 100 assays |
|---|---|
| Method | Spectrophotometric at 340nm |
| Total Assay Time | ~ 90 min |
| Detection Limit | 1-100% of sample weight |
| Application Examples | Cereal flours, food products and other materials |
| Method Recognition | AOAC (Method 996.11), AACC (Method 76-13.01), ICC (Standard |
Megazyme Total Starch HK Assay Kit, 100 assays
A modification of AOAC Method 996.11, AACC Method 76-13.01 and RACI Standard Method for the measurement, and analysis of total starch in cereal flours and food products. This kit contains an improved α-amylase that allows the amylase incubations to be performed at pH 5.0 (as well as pH 7.0). The method has been further modified by adjusting the D-glucose determination to a hexokinase/glucose-6-phosphate dehydrogenase/NADP+ based format.
UV-method for the determination of Total Starch in grains,
animal feeds, foodstuffs and other materials
Principle:
(α-amylase, 100°C + DMSO)
(1) Starch granules + H2O → maltodextrins
(amyloglucosidase)
(2) Maltodextrins + H2O → D-glucose
(hexokinase)
(3) D-Glucose + ATP → G-6-P + ADP
(glucose-6-phosphate dehydrogenase)
(4) G-6-P + NADP+ → gluconate-6-phosphate + NADPH + H+
Advantages
- Very competitive price (cost per test)
- All reagents stable for > 2 years after preparation
- Simple format
- Mega-Calc™ software tool is available from our website for hassle-free raw data processing
- Standard included
