Megazyme Fructanase Mixture (purified-powder)

SKU: E-FRMXPD

Powder (freeze dried)

Fructanase, Amyloglucosidase (E-AMGDF) and Isoamylase (E-ISAMY) are used in the enzyme hydrolysis step of two validated methods for the determination of polydextrose (a low molar mass dietary fiber) in foods: AOAC method 2000.11 and Chinese GB Standard 5009.245-2016.

EC 3.2.1.7
CAZy Family: GH32 

EC 3.2.1.80
CAZy Family: GH32 

Components: exo-inulinase 20,000 U (on kestose, at 40oC), endo-inulinase 1000 U (on fructan at 40oC), α-galactosidase 0.70 U (on p-nitrophenyl α-galactoside at 40oC), β-Glucanase 1.25 U (on β-glucan at 40oC) and pectinase 5.5 U (on pectin at 40oC).

Note: This product has been purified to remove most of the contamination with α-galactosidase, β-glucanase and pectinase which can interfere with the use of the preparation in the measurement of fructan, or in the solubilisation of “insoluble” fructan in the AOAC Total Dietary Fiber method. In this product, the contamination of exo-inulinase by α-galactosidase, β-glucanase and pectinase is 0.003%, 0.005% and 0.022%, respectively. In Fructozyme (NOVO), the contamination by α-galactosidase, β-glucanase and pectinase is 15.2%, 0.3% and 2% respectively.

Stability: Minimum 1 year at < -10oC. Check vial for details.

Content/size

20,000 units

Form

Powder (freeze dried)

Stability

Minimum 1 year at < -10oC. Check vial for details.

Specific activity

exo-inulinase 20,000 U (on kestose, at 40oC)
endo-inulinase 1000 U (on fructan at 40oC)
α-galactosidase 0.70 U (on p-nitrophenyl α-galactoside at 40oC)
β-Glucanase 1.25 U (on β-glucan at 40oC)
pectinase 5.5 U (on pectin at 40oC)

Megazyme Fructanase Mixture (purified-powder) (E-FRMXPD) Data Booklet

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