Megazyme Fructan Assay Kit, 100 assays

The Fructan Assay Kit is suitable for the specific measurement of fructan in plant extracts, animal feed and food products containing starch, sucrose and other sugars. It is used in three validated methods for the determination of fructan: AOAC method 999.03 (foods), AOAC method 2018.07 (Animal Feed) and AOAC method 2016.14 (infant formula and adult nutritionals).

New, improved procedure.

In the most recent development, a recombinant endo-levanase has been incorporated into the fructanase mixture, extending the use of the method to the measurement of levan-type fructans as are present in grasses such as timothy, cocksfoot, ryegrass and red fescue.

The method described in this booklet employs ultra-pure, recombinant enzymes and specifically measures fructans including inulin-type fructans from chicory, dahlia, jerusalem artichoke; highly branched fructans from onion and wheat stems and leaves; and levan-type fructans from pasture grasses such as timothy grass. The enzymes employed are completely devoid of contaminating enzymes active on β-glucan or gluco-oligosaccharides.

Colourimetric method for the determination of Fructan in plant products, foods, infant formula, animal feed and other materials

Principle:
(sucrase)
(1) Sucrose + H₂O → D-glucose + D-fructose

(β-amylase + maltase + pullulanase)
(2) Starch + maltosaccharides + H₂O → D-glucose

(borohydride)
(3) D-Glucose + D-fructose → D-sorbitol + D-mannitol
(non-reducing)

(exo-inulinase + endo-inulinase + endo-levanase)
(4) Fructan + H₂O → D-glucose + D-fructose

(100°C, 6 min)
(5) D-Glucose + D-fructose + PAHBAH → PAHBAH colour complex

Advantages

• Very cost effective
• All kit reagents stable for > 2 years after preparation
• Unaffected by high sucrose / reducing sugar concentrations
• Fructan kits are only available from Megazyme
• Simple format
• Mega-Calc™ software tool is available from our website for hassle-free raw data processing
• Standard included

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