Megazyme Digestible and Resistant Starch Assay Kit

Megazyme Available Carbohydrates/Dietary Fiber Assay Kit, 100 assays

SKU: K-ACHDF

The Available Carbohydrates/Dietary Fiber test kit is an integrated procedure for the measurement and analysis of available carbohydrates and dietary fiber in cereal products, fruit and vegetables and food products.

 

An integrated procedure for the measurement of Available
Carbohydrates and Dietary Fiber in cereal products, fruit and
vegetables and food products

Principle (Dietary Fiber):
(α-amylase + amyloglucosidase)
(1) Starch + H2O → glucose

(protease)
(2) Protein + H2O → peptides

(3) Dietary fiber determined gravimetrically following
alcohol precipitation

Principle (Available Carbohydrates):
(sucrase / maltase + β-galactosidase)
(4) Sucrose, maltose and lactose → D-glucose + D-fructose
+ D-galactose

(PGI, hexokinase + glucose-6-phosphate dehydrogenase)
(5) D-Glucose + D-fructose + ATP + NADP+ → gluconate-6-phosphate
+ NADPH + ADP + H+

 

Advantages

  • Very cost effective
  • All reagents stable for > 2 years after preparation
  • High purity / standardised enzymes employed
  • Only kit available
  • Mega-Calc™ software tool is available from our website for hassle-free raw data processing
  • Simple format
Kit Size

100 assays
Application Examples

Food ingredients, food products and other materials
Method Recognition

Dietary Fibre – AOAC (Methods 985.29, 991.42, 991.43 and 993.19)
and AACC (Methods 32-05.01, 32-07.01 and 32-21.01)

Megazyme Available Carbohydrates/Dietary Fiber Assay Kit (K-ACHDF) Data Booklet

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