| Enzyme Activity | exo-Polygalacturonase |
|---|---|
| EC Number | 3.2.1.82 |
| CAZy Family | GH28 |
| CAS Number | 37288-58-7 |
| Synonyms | exo-poly-alpha-galacturonosidase |
| Source | Yersinia enterocolitica |
| Concentration | Supplied at ~ 1,000 U/mL |
| Expression | Recombinant from Yersinia enterocolitica |
| Specificity | Hydrolysis of pectic acid from the non-reducing end, releasing digalacturonate. |
| Specific Activity | ~ 80 U/mg protein (60oC, pH 6.0 on Polygalacturonic Acid) |
| Unit Definition | One Unit of exo-polygalacturonase activity is defined as the amount of enzyme required to release one µmole of reducing-sugar equivalents per minute from Polygalacturonic Acid (10 mg/mL) in potassium phosphate buffer (100 mM), pH 6.0. |
| Temperature Optima | 60oC |
| pH Optima | 6 |
| Application examples | For use in research. |
Megazyme exo-Polygalacturonase (Yersinia enterocolitica)
High purity recombinant exo-polygalacturonase (Yersinia enterocolitica) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
| Content: | 500 Units at 60oC |
| Shipping Temperature: | Ambient |
| Storage Temperature: | 2-8oC |
| Formulation: | In 3.2 M ammonium sulphate |
| Physical Form: | Suspension |
| Stability: | > 4 years at 4oC |
