| Kit Size | 100 / 200 assays |
|---|---|
| Method | Spectrophotometric at 400nm |
| Reaction Time | ~ 30min |
| Detection Limit | 0.05 U/mL |
| Application Examples | Cereal flours, fermentation broths and other materials |
| Method Recognition | AOAC (Method 2002.01), AACC (Method 22-02.01), ICC (Standard No. 303), RACI (Standard Method) and CCFRA (Flour Testing Working Group Method 0018) |
Megazyme α-Amylase Assay Kit (Ceralpha Method)
The Ceralpha Method: α-Amylase test kit is suitable for the specific measurement and analysis of α-amylase in cereal grains and fermentation broths (fungal and bacterial).
Colourimetric method for the determination of α-Amylase
in foodstuffs, feed and fermentation products
Principle:
(α-amylase)
(1) Benzylidene-G7-α-PNP + H2O → Benzylidene-GX + G(7-X)-α-PNP
(thermostable α-glucosidase)
(2) G(7-X)-α-PNP + H2O → D-glucose + PNP
(alkaline solution)
(3) PNP → phenolate ion (yellow colour)
Note: PNP = 4-nitrophenol
Advantages
- Very cost effective
- All reagents stable for > 2 years after preparation
- Very specific
- Simple format
- Mega-Calc™ software tool is available from our website for hassle-free raw data processing
- Standard included
